ISSN: 2450-8330
OAI
DOI: 10.18276/ab.2017.24-01
Liste der Ausgaben /
No. 24
Assessment of genetic variability in common whitefish from the catchment area of the Oder river using microsatellite markers
(Ocena zmienności genetycznej siei ze zlewni Odry z wykorzystaniem markerów mikrosatelitarnych)
| Autoren: |
Magdalena
Achrem
University of Szczecin, Faculty of Biology, Department of Cell Biology, Molecular Biology and Biotechnology Center Lidia Skuza University of Szczecin, Faculty of Biology, Department of Cell Biology, Molecular Biology and Biotechnology Center Lucyna Kirczuk University of Szczecin, Faculty of Biology, Department of General Zoology Józef Domagała University of Szczecin, Faculty of Biology, Department of General Zoology Małgorzata Pilecka-Rapacz University of Szczecin, Faculty of Biology, Department of General Zoology Robert Czerniawski University of Szczecin, Faculty of Biology, Department of General Zoology |
| Schlüsselbegriffe: | Coregonus maraena SSR genetic variability |
| Veröffentlichungsdatum der gesamten Ausgabe: | 2017 |
| Seitenanzahl: | 9 (5-13) |
Abstract
Common whitefish (Coregonus maraena) in Poland belongs to the endangered species.
The degradation of the environment causes common whitefish to lose its natural reproduction
sites. The natural genetic structure of whitefish has been compromised by anthropogenetic
activities involving eutrophication, river regulation, the introduction of non-native species
and as well as excessive exploitation of the species. The genetic variability of common
whitefish (Coregonus maraena) from 2 sites: Pomeranian Bay and the lower Oder river,
was assessed using microsatellite markers. A total of 45 caught individuals were analysed
(26 from Pomeranian Bay and 19 from the Oder river). Polymorphism at nine loci, Str60INRA,
Str73INRA, Strutta 12, OmyFgt1TUF, Str85INRA, Str591INRA, Ssa85, Ssa197, T3-13 was
assessed. The results indicated that all the investigated populations showed a high level of
genetic variability. The level of genetic variability was determined using the FST parameter
and was high investigated populations (0.215). Microsatellite analysis demonstrated a higher
observed heterozygosity as compared with the expected heterozygosity in all the investigated
populations. The FIS coefficient values below zero in all the investigated populations of common
whitefish indicate the excess of heterozygotes. The high number of heterozygotes may be
related with a more intense influx of genes from outside of the local population. The study
demonstrated that microsatellite markers (SSR) are very useful in the assessment of the genetic
variability of common whitefish (Coregonus maraena). Our results characterize the selected
populations of whitefish and may be useful for further research on this endangered species.
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