Central European Journal of Sport Sciences and Medicine

ISSN: 2300-9705     eISSN: 2353-2807    OAI    DOI: 10.18276/cej.2020.4-05
CC BY-SA   Open Access   DOAJ  DOAJ

Lista wydań / Vol. 32, No. 4/2020
Nutritional profile and oxidative stress in adolescent soccer players

Autorzy: Francine Milani Guissoni
Laboratory of Nutrition and Metabolism, Faculty of Medicine of Ribeirão Preto. University of Sao Paulo (USP)

Rafael Deminice
Department of Physical Education. Faculty of Physical Education and Sport - State University of Londrina, Brazil

Paula Payão Ovidio
Laboratory of Nutrition and Metabolism, Faculty of Medicine of Ribeirão Preto. University of Sao Paulo (USP)

Edson Zangiacomi Martinez
Department of Social Medicine. Faculty of Medicine of Ribeirão Preto. University of Sao Paulo (USP)

Alceu Afonso Jordao
Laboratory of Nutrition and Metabolism, Faculty of Medicine of Ribeirão Preto. University of Sao Paulo (USP)

Mirele S. Mialich
University of Sao Paulo (USP), Ribeirão Preto Medical School, Department of Health Sciences, Brazil
Słowa kluczowe: adolescent exercise nutritional status oxidative stress soccer
Data publikacji całości:2020
Liczba stron:9 (51-59)
Cited-by (Crossref) ?:


High-intensity exercise increases reactive oxygen species formation, which in excess may cause oxidative stress. We assessed nutritional status and exercise-induced oxidative stress in 20 adolescent male soccer players (age: 15-17). Participants were divided into two teams for a 60-minute friendly match and evaluated immediately before (Pre-match), 30 minutes after (Post-match I) and 24 hours after (Post-match II) the game. All players recorded a 3-day dietary intake. Biochemical tests were performed for lipid profile, muscle damage (creatinine and creatinine kinase [CK]) and oxidative stress (thiobarbituric acid-reactive substances [TBARS], protein carbonyls [PC], reduced glutathione [GSH], and vitamins E, C, and A). CK and creatinine were significantly elevated at Post-match I (p < 0.01), returning to baseline at Post-match II. Vitamins E, C and A were significantly elevated at Post-match I (p < 0.01), but only vitamins E and A remained high at Post-match II. TBARS showed no significant changes. GSH showed a significant decrease (p < 0.01) and PC showed a slight but significant increase (p < 0.01) at Post-match II. The recruitment of non-enzymatic antioxidants prevented lipid peroxidation, but dietary and especially endogenous defence responses were insufficient to prevent protein oxidation. Proper nutrition is essential to improve the activity of the antioxidant defence system, preventing exercise-induced oxidative stress.
Pobierz plik

Plik artykułu


1.Ascensão, A., Rebelo, A., Oliveira, E., Marques, F., Pereira, L., Magalhães, J. (2008). Biochemical impact of a soccer match  analysis of oxidative stress and muscle damage markers throughout recovery. Clinical Biochemistry, 41, 841-51. doi: 10.1016/j.clinbiochem.2008.04.008.
2.Andersson, H., Karlsen, A., Blomhoff, R., Raastad, T., Kadi, F. (2010). Plasma antioxidant responses and oxidative stress following a soccer game in elite female players. Scandinavian Journal of Medicine and Science in Sports, 20, 600-608. doi: 10.1111/j.1600-0838.2009.00987.x.
3.Arent, S.M., Pellegrino, J.K., Williams, C.A., Difabio, D.A., Greenwood, J.C. (2010). Nutritional supplementation, performance, and oxidative stress in college soccer players. Journal of Strength and Conditioning Research, 24, 1117-1124. doi: 10.1519/JSC.0b013e3181cb70b8.
4.Arnaud, J., Fortis, I., Blachier, S., Kia, D., Favier, A. (1991). Simultaneous determination of retinol, alpha-tocopherol and beta-carotene in serum by isocratic high-performance liquid chromatography. Journal of Chromatography, 572, 103-116. doi: 10.1016/0378-4347(91)80476-S.
5.Bessey, O.A. (1960). Ascorbic acid. Microchemical methods. In: P. Gyorgy (ed.), Vitamin Methods (p. 303). New York: Academic Press.
6.Bloomer, R.J., Goldfarb, A.H., McKenzie, M.J. (2006). Oxidative stress response to aerobic exercise: comparison of antioxidant supplements. Medicine and Science in Sports and Exercise, 38, 1098-1105. doi: 10.1249/01.mss.0000222839.51144.3e.
7.Boisseau, N., Vermorel, M., Rance, M., Duché, P., Patureau-Mirand, P. (2007). Protein requirements in male adolescent soccer players. European Journal of Applied Physiology, 100, 27-33. doi: 10.1007/s00421-007-0400-4.
8.de Oliveira, D.C.X., Rosa, F.T., Simões-Ambrósio, L., Jordao, A.A., Deminice, R. (2019). Antioxidant vitamin supplementation prevents oxidative stress but does not enhance performance in young football athletes. Nutrition, 63-64, 29-35. doi: 10.1016/j.nut.2019.01.007.
9.Eskici, G. (2016). The Importance of Vitamins for Soccer Players. International Journal Vitamins Nutrition and Research, May 10, 1-21.
10.Estimated Average Requirement (EAR) (2000). Dietary References Intakes (DRIs).
11.Estimated Average Requirement (EAR) (2001). Dietary References Intakes (DRIs).
12.Fatouros, I.G., Chatzinikolaou, A., Douroudos, I.I., Nikolaidis, M.G., Kyparos, A., Margonis, K., Michailidis, Y., Vantarakis, A., Taxildaris, K., Katrabasas, I., Mandalidis, D., Kouretas, D., Jamurtas, A.Z. (2010). Time-course of changes in oxidative stress and antioxidant status responses following a soccer game. Journal of Strength and Conditioning Research, 24, 3278-3286. doi: 10.1519/JSC.0b013e3181b60444.
13.Galanti, G., Stefani, L., Scacciati, I., Mascherini, G., Buti, G., Maffulli, N. (2014). Eating and nutrition habits in young competitive athletes: a comparison between soccer players and cyclists. Transl Medicina @ UniSa, 19 (11), 44-7. eCollection.
14.González, J.A., Cobos, I.H., Molina, E.S. (2010). Estrategias nutricionales para la competición en el fútbol [Nutritional strategies for soccer playing]. Revista Chilena de Nutrición, 37, 118-122. doi: 10.4067/S0717-75182010000100012.
15.Gravina, L., Ruiz, F., Lekue, J.A., Irazusta, J., Gil, S.M. (2011). Metabolic impact of a soccer match on female players. Journal of Sports Sciences, 29, 1345-1352. doi: 10.1080/02640414.2011.597420.
16.Hadžović-Džuvo, A., Valjevac, A., Lepara, O., Pjanić, S., Hadžimuratović, A., Mekić, A. (2014) Oxidative stress status in elite athletes engaged in different sport disciplines. Bosnian Journal of Basic Medical Science, 14, 56-62.
17.Hernandez, A.J., Nahas, R.M. (2009). Modificações dietéticas, reposição hídrica, suplementos alimentares e drogas: comprovação de ação ergogênica e potenciais riscos para a saúde [Dietary changes, water replacement, food supplements and drugs: evidence of ergogenic action and potential health risks]. Revista Brasileira de Medicina do Esporte, 15, 2-12. doi: 10.1590/S1517-86922009000400001.
18.Hespel, P., Maughan, R.J., Greenhaff, P.L. (2006). Dietary supplements for football. Journal of Sports Sciences, 24, 749-761. doi: 10.1080/02640410500482974.
19.Iglesias-Gutiérrez, E., García-Rovés, P.M., Rodríguez, C., Braga, S., García-Zapico, P., Patterson, A.M. (2005). Food habits and nutritional status assessment of adolescent soccer players. A necessary and accurate approach. Canadian Journal of Applied Physiology, 30, 18-32. doi: 10.1139/h05-102.
20.Iglesias-Gutiérrez, E., García, A., García-Zapico, P., Pérez-Landaluce, J., Patterson, A.M., García-Rovés, P.M. (2012). Is there a relationship between the playing position of soccer players and their food and macronutrient intake? Applied Physiology, Nutrition, and Metabolism, 37, 225-232. doi: 10.1139/h11-152.
21.Jelliffe, D.B. (1966). The assessment of the nutritional status of the community (with special reference to field surveys in developing regions of the world). Monograph series – World Health Organization, 53, 3-271. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/4960818 (17.12.2019).
22.Karakilcik, A.Z., Halat, R., Zerin, M., Celik, H., Nazligul, Y. (2014). Effects of vitamin C and exercise on lipid profile, platelet and erythrocyte indices in young soccer players. Journal of Sports Medicine and Physical Fitness, 54, 665-71.
23.Levine, R.L., Williams, J.A., Stadtman, E.R., Shacter, E. (1994). Carbonyl assays for determination of oxidatively modified proteins. Methods in Enzymology, 233, 346-357. doi: 10.1016/S0076-6879(94)33040-9.
24.Maughan, R.J., Bartagi, Z., Dvorak, J., Zerguini, Y. (2008). Dietary intake and body composition of football players during the holy month of Ramadan. Journal of Sports Sciences, 26, S29-S38. doi: 10.1080/02640410802409675.
25.Mello, R., Mello, R., Gomes, D., Paz, G.A., Nasser, I., Miranda, H., Salerno, V.P. (2017). Oxidative stress and antioxidant biomarker responses after a moderate-intensity soccer training session. Res Sports Med., 25 (3), 322-332.
26.Michailidis, Y., Jamurtas, A.Z., Nikolaidis, M.G., Fatouros, I.G., Koutedakis, Y., Papassotiriou, I., Kouretas, D. (2007). Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress. Medicine and Science in Sports and Exercise, 39, 1107-1113. doi: 10.1249/01.mss.0b013e318053e7ba.
27.Nieman, D.C., Bishop, N.C. (2006). Nutritional strategies to counter stress to the immune system in athletes, with special reference to football. Journal of Sports Sciences, 24, 763-772. doi: 10.1080/02640410500482982.
28.Nikolaidis, P.T., Vassilios Karydis, N. (2011). Physique and body composition in soccer players across adolescence. Asian Journal of Sports Medicine, 2, 75-82. doi: 10.5812/asjsm.34782.
29.Noda, Y., Iide, K., Masuda, R., Kishida, R., Nagata, A., Hirakawa, F., Yoshimura, Y., Imamura, H. (2009). Nutrient intake and blood iron status of male collegiate soccer players. Asia Pacific Journal of Clinical Nutrition, 18, 344-350. Retrieved from: http://www.ncbi.nlm.nih.gov/pubmed/19786382 (15.12.2019).
30.Powers, S.K., Radak, Z., Ji, L.L. (2016). Exercise-induced oxidative stress: past, present and future. Journal of Physiology, Feb 19.
31.Regulamento Técnico para Fixação de Identidade e Qualidade de Alimentos para Praticantes de Atividade Física [Technical Regulations for Establishment of Identity and Quality of Food for People Performing Physical Activity] (1998). Brazilian Health Surveillance Agency, Ordinance No. 222 (March 24).
32.Roehrs, M., Valentini, J., Bulcão, R., Moreira, J.C., Biesalski, H., Limberger, R.P., Garcia, S.C. (2009). The plasma retinol levels as pro-oxidant/oxidant agents in haemodialysis patients. Nephrology, Dialysis, Transplantation, 24, 2212-2218. doi: 10.1093/ndt/gfp001.
33.Russell, M., Pennock, A. (2011). Dietary analysis of young professional soccer players for 1 week during the competitive season. Journal of Strength and Conditioning Research, 25, 1816-1823. doi: 10.1519/JSC.0b013e3181e7fbdd.
34.Santos, R.D., Pereira, A., Duncan, B.B., Polanczyk, C.A., Moriguchi, E.H., Rosito, G.A., Bodanese, L.C., Clausell, N.O. (2001). III Diretrizes Brasileiras sobre dislipidemias e diretriz de prevenção da aterosclerose do Departamento de Aterosclerose da Sociedade Brasileira de Cardiologia. Arquivos Brasileiros de Cardiologia, 77, 1-48. doi: 10.1590/S0066-782X2001001500001.
35.Sedlak J., Lindsay, R.H. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Analytical Biochemistry, 25, 192-205. doi: 10.1016/0003-2697(68)90092-4.
36.Shing, C.M., Peake, J.M., Ahern, S.M., Strobel, N.A., Wilson, G., Jenkins, D.G., Coombes, J.S. (2007). The effect of consecutive days of exercise on markers of oxidative stress. Applied Physiology, Nutrition, and Metabolism, 32, 677-685. doi: 10.1139/H07-051.
37.Silva, J.R., Rebelo, A., Marques, F., Pereira, L., Seabra, A., Ascensão, A., Magalhães, J. (2014). Biochemical impact of soccer: an analysis of hormonal, muscle damage, and redox markers during the season. Applied Physiology Nutrition and Metabolism, 39, 432-8.
38.Souglis, A., Bogdanis, G.C., Chryssanthopoulos, C., Apostolidis, N., Geladas, N.D. (2018). Time Course of Oxidative Stress, Inflammation, and Muscle Damage Markers for 5 Days After a Soccer Match: Effects of Sex and Playing Position. J Strength Cond Res., 32 (7), 2045-2054. doi: 10.1519/JSC.0000000000002436.
39.Spirlandeli, A.L., Deminice, R., Jordao, A.A. (2014). Plasma malondialdehyde as biomarker of lipid peroxidation: effects of acute exercise. International Journal of Sports Medicine, 35, 14-8. doi: 10.1055/s-0033-1345132.
40.Tahara, Y., Moji, K., Tsunawake, N., Fukuda, R., Nakayama, M., Nakagaichi, M., Nakagaichi, M., Komine, T., Kusano, Y., Aoyagi, K. (2006). Physique, body composition and maximum oxygen consumption of selected soccer players of Kunimi High School, Nagasaki, Japan. Journal of Physiological Anthropology, 25, 291-297. doi: 10.2114/jpa2.25.291.
41.Tauler, P., Ferrer, M.D., Sureda, A., Pujol, P., Drobnic, F., Tur, J.A., Pons, A. (2008). Supplementation with an antioxidant cocktail containing coenzyme Q prevents plasma oxidative damage induced by soccer. European Journal of Applied Physiology, 104, 777-785. doi:10.1007/s00421-008-0831-6.
42.Zanella, A.M., Souza, D.R.S., Godoy, M.F. (2007). Influência do exercício físico no perfil lipídico e estresse oxidativo. Arquivos de Ciência da Saúde, 14, 107-112. Retrieved from: https://pesquisa.bvsalud.org/portal/resource/pt/lil-490338 (02.11.2020).
43.Zoppi, C.C., Hohl, R., Silva, F.C., Lazarim, F.L., Neto, J.M., Stancanneli, M., Macedo, D.V. (2006). Vitamin C and E supplementation effects in professional soccer players under regular training. Journal of the International Society of Sports Nutrition, 3, 3744. doi:10.1186/1550-2783-3-2-37.