Central European Journal of Sport Sciences and Medicine

ISSN: 2300-9705     eISSN: 2353-2807    OAI    DOI: 10.18276/cej.2025.4-06
CC BY-SA   Open Access   DOAJ  DOAJ

Issue archive / Vol. 48, No. 4/2024
Effect of creatine and caffeine supplementation together and separately on serum lactate leveles and performance of male wrestlers

Authors: Seyed Mohammad Seyedin Koojan ORCID
Physical Activity and Health Promotion Course, Faculty of Medicine and Surgery, University of Rome Tor Vergata, Italy

Giuseppe Merra ORCID
Department of Biomedicine and Prevention, University of Rome Tor Vergata, Italy

Giulia Frank ORCID
PhD School of Applied Medical-Surgical-Sciences, Univeristy of Rome Tor Vergata, Italy

Paola Gualtieri ORCID
Department of Biomedicine and Prevention, Section of Clinical Nutrition and Nutrigenomics, Univeristy of Rome Tor Vergata, Italy

Mahsa Farahani ORCID
Physical Activity and Health Promotion Course, Faculty of Medicine and Surgery, University of Rome Tor Vergata, Italy

Martin Hofmeister ORCID
Department Food and Nutrition, Munich, Consumer Centre of the German Federal State of Bavaria, Germany

David Della Morte Canosci ORCID
Physical Activity and Health Promotion Course, Faculty of Medicine and Surgery, University of Rome Tor Vergata, Italy

Fateme Shabkhiz ORCID
Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran

Laura Di Renzo ORCID
Department of Biomedicine and Prevention, Section of Clinical Nutrition and Nutrigenomics, Univeristy of Rome Tor Vergata, Italy
Keywords: caffeine creatine sport performance sports nutrition wrestling
Whole issue publication date:2025-03-20
Page range:12 (57-68)
Klasyfikacja JEL: I10
Cited-by (Crossref) ?:

Abstract

Supplementation is widely used by athletes to enhance physical capabilities, improve exercise performance, and achieve other ergogenic effects. These practices promote adaptability, recovery, and the ability to train and compete. The aim of this study was to investigate the effects of caffeine, creatine, and their combination on performance and blood lactate levels in semi-professional male wrestlers. A total of twenty wrestlers participated in a double-blind, crossover, randomized placebo- controlled study, which included five experimental treatments: creatine (Cr), caffeine (CAF), creatine + caffeine (Cr+CAF), control (Con), and placebo (PLA). The participants, with an average age of 19.43 ±1.75 and a body mass index of 23.11 ±3.34, were selected voluntarily. Creatine and caffeine powder were mixed in water and consumed 60 minutes prior to each evaluation session. Performance and blood lactate values were measured before and immediately after the Dummy throw test. The results showed that performance significantly improved following the Cr+CAF and Cr conditions (p ≤ 0.05). Additionally, blood lactate values immediately after the Dummy throw test were significantly lower in the Cr condition (P = 0.04) compared to PLA and CON. However, although blood lactate values decreased in the Cr+CAF condition, the difference was not statistically significant (P > 0.05). In conclusion, male wrestlers may benefit from the ergogenic effects of creatine and caffeine when consumed separately or in combination.
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Bibliography

1.Aaserud, R., Gramvik, P., Olsen, S. R., & Jensen, J. (1998). Creatine supplementation delays onset of fatigue during repeated bouts of sprint running. Scandinavian Journal of Medicine & Science in Sports, 8(5), 247–251.
2.Aedma, M., Timpmann, S., Lätt, E., Ööpik, V. (2015). Short-term creatine supplementation has no impact on upper-body anaerobic power in trained wrestlers. J Int Soc Sports Nutr, 12–45. https://doi.org/10.1186/s12970-015-0107-6
3.Ahmun, R., Tong, R. J., & Grimshaw, P. N. (2005). The effects of acute creatine supplementation on multiple sprint cycling and running performance in rugby players (Doctoral dissertation, National Strength & Conditioning Association).
4.Anioł-Strzyżewska, K., & Starosta, W. (2012). The evaluation of specific effort of the highly advanced wrestlers in the many years training cycle [Ocena specyficznej wydolności wysoko zaawansowanych zapaśników w wieloletnim cyklu treningowym] Sci. J. Grad. Sch. Phys. Educ. Tour. Bialystok, 8, 110–112.
5.Balsom, P. D., Ekblom, B., Söerlund, K., Sjödln, B., & Hultman, E. (1993). Creatine supplementation and dynamic high‐intensity intermittent exercise. Scandinavian Journal of Medicine & Science in Sports, 3(3), 143–149.
6.Balsom, P. D., Söderlund, K., Sjödin, B., & Ekblom, B. (1995). Skeletal muscle metabolism during short duration high‐intensity exercise: influence of creatine supplementation. Acta Physiologica Scandinavica, 154(3), 303–310.
7.Bangsbo, J. (2003). Physiology of training. Science and Soccer, 2, 47–58.
8.Barbas, I., Fatouros, I. G., Douroudos, I. I., Chatzinikolaou, A., Michailidis, Y., Draganidis, D., Jamurtas, A. Z., Nikolaidis, M. G., Parotsidis, C., Theodorou, A. A., Katrabasas, I., Margonis, K., Papassotiriou, I., & Taxildaris, K. (2011). Physiological and performance adaptations of elite Greco-Roman wrestlers during a one-day tournament. European journal of applied physiology, 111(7), 1421– 1436. https://doi.org/10.1007/s00421-010-1761-7
9.Bemben, M. G., & Lamont, H. S. (2005). Creatine supplementation and exercise performance. Sports Medicine, 35(2), 107–125.
10.Bogdanis, G. C., Nevill, M. E., Boobis, L. H., & Lakomy, H. K. (1996). Contribution of phosphocreatine and aerobic metabolism to energy supply during repeated sprint exercise. Journal of Applied Physiology, 80(3), 876–884.
11.Branch, J. D. (2003). Effect of creatine supplementation on body composition and performance: a meta-analysis. International Journal of Sport Nutrition and Exercise Metabolism, 13(2), 198–226.
12.Burke, L., Cort, M., Cox, G., Crawford, R., Desbrow, B., Farthing, L., and et al., (2006). Supplements and sports foods. Clinical Sports Nutrition, 3.
13.Casey, A., Constantin-Teodosiu, D., Howell, S., Hultman, E. G. P. L., & Greenhaff, P. L. (1996). Creatine ingestion favorably affects performance and muscle metabolism during maximal exercise in humans. American Journal of Physiology-Endocrinology And Metabolism, 271(1), E31–E37.
14.Ceddia, R. B., & Sweeney, G. (2004). Creatine supplementation increases glucose oxidation and AMPK phosphorylation and reduces lactate production in L6 rat skeletal muscle cells. The Journal of Physiology, 555(2), 409–421.
15.Chaabene, H., Negra, Y., Bouguezzi, R., Mkaouer, B., Franchini, E., Julio, U., & Hachana, Y. (2017). Physical and Physiological Attributes of Wrestlers: An Update. Journal of strength and conditioning research, 31(5), 1411–1442. https://doi.org/10.1519/JSC.0000000000001738
16.Cooper, R., Naclerio, F., Allgrove, J., & Jimenez, A. (2012). Creatine supplementation with specific view to exercise/sports performance: an update. Journal of the International Society of Sports Nutrition, 9(1), 1–11.
17.Cornish, R. S., Bolam, K. A., & Skinner, T. L. (2015). Effect of caffeine on exercise capacity and function in prostate cancer survivors. Medicine and Science in Sports and Exercise, 47(3), 468–475.
18.Demirkan, E., Koz, M., Kutlu, M., & Favre, M. (2015). Comparison of physical and physiological profiles in elite and amateur young wrestlers. [Comparative study]. Journal of Strength and Conditioning Research, 29(7), 1876–1883. https://doi.org/10.1519/JSC.0000000000000833
19.Durkalec-Michalski, K., Nowaczyk, P. M., Główka, N., & Grygiel, A. (2019). Dose-dependent effect of caffeine supplementation on judo-specific performance and training activity: a randomized placebo-controlled crossover trial. Journal of the International Society of Sports Nutrition, 16(1), 1–14.
20.Forbes, S. C., & Candow, D. G. (2018). Timing of creatine supplementation and resistance training: A brief review. Journal of Exercise and Nutrition, 1(5).
21.Fredholm, B. B., Bättig, K., Holmén, J., Nehlig, A., & Zvartau, E. E. (1999). Actions of caffeine in the brain with special reference to factors that contribute to its widespread use. Pharmacological Reviews, 51(1), 83–133.
22.Fukuda, D. H., Kendall, K. L., Smith, A. E., Dwyer, T. R., & Stout, J. R. (2011). The development of physiological profiles and identification of training needs in NCAA female collegiate rowers using isoperformance curves. European journal of applied physiology, 111(4), 679–685. https://doi.org/10.1007/s00421-010-1683-4
23.Glaister, M., Williams, B. H., Muniz-Pumares, D., Balsalobre-Fernández, C., & Foley, P. (2016). The effects of caffeine supplementation on physiological responses to submaximal exercise in endurance-trained men. PLoS One, 11(8), e0161375.
24.Goldstein, E. R., Ziegenfuss, T., Kalman, D., Kreider, R., Campbell, B., Wilborn, C., Taylor, L., Willoughby, D., Stout, J., Graves, B. S., Wildman, R., Ivy, J. L., Spano, M., Smith, A. E., & Antonio, J. (2010). International society of sports nutrition position stand: caffeine and performance. Journal of the International Society of Sports Nutrition, 7(1), 5. https://doi.org/10.1186/1550-2783-7-5
25.Gonzalez, A. M., Walsh, A. L., Ratamess, N. A., Kang, J., & Hoffman, J. R. (2011). Effect of a pre-workout energy supplement on acute multi-joint resistance exercise. Journal of Sports Science & Medicine, 10(2), 261.
26.Graef, J. L., Smith, A. E., Kendall, K. L., Fukuda, D. H., Moon, J. R., Beck, T. W., Cramer, J. T., & Stout, J. R. (2009). The effects of four weeks of creatine supplementation and high-intensity interval training on cardiorespiratory fitness: a randomized controlled trial. Journal of the International Society of Sports Nutrition, 6, 18. https://doi.org/10.1186/1550-2783-6-18
27.Graham, T. E. (2001). Caffeine, coffee and ephedrine: impact on exercise performance and metabolism. Canadian Journal of Applied Physiology, 26(S1), S186–S191.
28.Graham, T. E., Helge, J. W., MacLean, D. A., Kiens, B., & Richter, E. A. (2000). Caffeine ingestion does not alter carbohydrate or fat metabolism in human skeletal muscle during exercise. The Journal of Physiology, 529(Pt 3), 837.
29.Greydanus, D. E., & Patel, D. R. (2010). Sports doping in the adolescent: the Faustian conundrum of Hors de Combat. Pediatric Clinics, 57(3), 729–750.
30.Harris, R. C., Söderlund, K., & Hultman, E. (1992). Elevation of creatine in resting and exercised muscle of normal subjects by creatine supplementation. Clinical Science, 83(3), 367–374.
31.Hespel, P., Op‘t Eijnde, B., & Van Leemputte, M. (2002). Opposite actions of caffeine and creatine on muscle relaxation time in humans. Journal of Applied Physiology, 92(2):513–8. https://doi.org/10.1152/japplphysiol.00255.2001
32.Hoffman, J. R., Ratamess, N. A., Ross, R., Shanklin, M., Kang, J., & Faigenbaum, A. D. (2008). Effect of a pre-exercise energy supplement on the acute hormonal response to resistance exercise. Journal of strength and conditioning research, 22(3), 874–882. https://doi.org/10.1519/JSC.0b013e31816d5db6
33.Kedia, A. W., Hofheins, J. E., Habowski, S. M., Ferrando, A. A., Gothard, M. D., & Lopez, H. L. (2014). Effects of a pre-workout supplement on lean mass, muscular performance, subjective workout experience and biomarkers of safety. International journal of medical sciences, 11(2), 116.
34.Kendall, K. L., Moon, J. R., Fairman, C. M., Spradley, B. D., Tai, C. Y., Falcone, P. H., Carson, L. R., Mosman, M. M., Joy, J. M., Kim, M. P., Serrano, E. R., & Esposito, E. N. (2014). Ingesting a preworkout supplement containing caffeine, creatine, β-alanine, amino acids, and B vitamins for 28 days is both safe and efficacious in recreationally active men. Nutrition research (New York, N.Y.), 34(5), 442–449. https://doi.org/10.1016/j.nutres.2014.04.003
35.Kocak, S., & Karli, U. (2003). Effects of high dose oral creatine supplementation on anaerobic capacity of elite wrestlers. Journal of Sports Medicine and Physical Fitness, 43(4), 488.
36.Kraemer, W. J., Hatfield, D. L., Spiering, B. A., Vingren, J. L., Fragala, M. S., Ho, J. Y., Volek, J. S., Anderson, J. M., & Maresh, C. M. (2007). Effects of a multi-nutrient supplement on exercise performance and hormonal responses to resistance exercise. European journal of applied physiology, 101(5), 637–646. https://doi.org/10.1007/s00421-007-0535-3
37.López-González, L. M., Sánchez-Oliver, A. J., Mata, F., Jodra, P., Antonio, J., & Domínguez, R. (2018). Acute caffeine supplementation in combat sports: a systematic review. Journal of the International Society of Sports Nutrition, 15(1), 1–11.
38.Lowery, R. P., Joy, J. M., Dudeck, J. E., Oliveira de Souza, E., McCleary, S. A., Wells, S., Wildman, R., & Wilson, J. M. (2013). Effects of 8 weeks of Xpand® 2X pre workout supplementation on skeletal muscle hypertrophy, lean body mass, and strength in resistance trained males. Journal of the International Society of Sports Nutrition, 10(1), 44. https://doi.org/10.1186/1550-2783-10-44
39.Magkos, F., & Kavouras, S. A. (2005). Caffeine use in sports, pharmacokinetics in man, and cellular mechanisms of action. Critical Reviews in Food Science and Nutrition, 45(7-8), 535–562.
40.Maughan, R. J., Burke, L. M., Dvorak, J., Larson-Meyer, D. E., Peeling, P., Phillips, S. M., Rawson, E. S., Walsh, N. P., Garthe, I., Geyer, H., Meeusen, R., van Loon, L., Shirreffs, S. M., Spriet, L. L., Stuart, M., Vernec, A., Currell, K., Ali, V. M., Budgett, R. G. M., Ljungqvist, A., … Engebretsen, L. (2018). IOC Consensus Statement: Dietary Supplements and the High-Performance Athlete. International journal of sport nutrition and exercise metabolism, 28(2), 104–125. https://doi.org/10.1123/ijsnem.2018-0020
41.Nawrot, P., Jordan, S., Eastwood, J., Rotstein, J., Hugenholtz, A., & Feeley, M. (2003). Effects of caffeine on human health. Food Additives & Contaminants, 20(1), 1–30.
42.Oopik, V., Paasuke, M., Timpmann, S., Medijainen, L., Ereline, J., & Gapejeva, J. (2002). Effects of creatine supplementation during recovery from rapid body mass reduction on metabolism and muscle performance capacity in well-trained wrestlers. Journal of Sports Medicine and Physical Fitness, 42(3), 330–339.
43.Ormsbee, M. J., Mandler, W. K., Thomas, D. D., Ward, E. G., Kinsey, A. W., Simonavice, E., Panton, L. B., & Kim, J. S. (2012). The effects of six weeks of supplementation with multi-ingredient performance supplements and resistance training on anabolic hormones, body composition, strength, and power in resistance-trained men. Journal of the International Society of Sports Nutrition, 9(1), 49. https://doi.org/10.1186/1550-2783-9-49
44.Pakulak, A., Candow, D. G., Totosy de Zepetnek, J., Forbes, S. C., & Basta, D. (2022). Effects of Creatine and Caffeine Supplementation During Resistance Training on Body Composition, Strength, Endurance, Rating of Perceived Exertion and Fatigue in Trained Young Adults. Journal of dietary supplements, 19(5), 587–602. https://doi.org/10.1080/19390211.2021.1904085
45.Persky, A. M., & Rawson, E. S. (2007). Safety of creatine supplementation. Sub-cellular biochemistry, 46, 275–289. https://doi. org/10.1007/978-1-4020-6486-9_14
46.Ratamess, N. A., Hoffman, J. R., Ross, R., Shanklin, M., Faigenbaum, A. D., & Kang, J. (2007). Effects of an amino acid/creatine energy supplement on the acute hormonal response to resistance exercise. International Journal of Sport Nutrition and Exercise Metabolism, 17(6), 608–623.
47.Schneiker, K. T., Bishop, D., Dawson, B., & Hackett, L. P. (2006). Effects of caffeine on prolonged intermittent-sprint ability in team-sport athletes. Medicine and Science in Sports and Exercise, 38(3), 578–585.
48.Shelmadine, B., Cooke, M., Buford, T., Hudson, G., Redd, L., Leutholtz, B., & Willoughby, D. S. (2009). Effects of 28 days of resistance exercise and consuming a commercially available pre-workout supplement, NO-Shotgun(R), on body composition, muscle strength and mass, markers of satellite cell activation, and clinical safety markers in males. Journal of the International Society of Sports Nutrition, 6, 16. https://doi.org/10.1186/1550-2783-6-16
49.Smith, A. E., Fukuda, D. H., Kendall, K. L., & Stout, J. R. (2010). The effects of a pre-workout supplement containing caffeine, creatine, and amino acids during three weeks of high-intensity exercise on aerobic and anaerobic performance. Journal of the International Society of Sports Nutrition, 7(1), 1–11.
50.Sökmen, B., Armstrong, L. E., Kraemer, W. J., Casa, D. J., Dias, J. C., Judelson, D. A., & Maresh, C. M. (2008). Caffeine use in sports: considerations for the athlete. Journal of strength and conditioning research, 22(3), 978–986. https://doi.org/10.1519/JSC.0b013e3181660cec
51.Spillane, M., Schwarz, N., Leddy, S., Correa, T., Minter, M., Longoria, V., & Willoughby, D. S. (2011). Effects of 28 days of resistance exercise while consuming commercially available pre- and post-workout supplements, NO-Shotgun® and NO-Synthesize® on body composition, muscle strength and mass, markers of protein synthesis, and clinical safety markers in males. Nutrition & metabolism, 8, 78. https://doi.org/10.1186/1743-7075-8-78
52.Spradley, B. D., Crowley, K. R., Tai, C. Y., Kendall, K. L., Fukuda, D. H., Esposito, E. N., and et al., (2012). Ingesting a pre-workout supplement containing caffeine, B-vitamins, amino acids, creatine, and beta-alanine before exercise delays fatigue while improving reaction time and muscular endurance. Nutrition & Metabolism, 9(1), 1–9.
53.Spriet, L. L., & Gibala, M. J. (2004). Nutritional strategies to influence adaptations to training. Journal of Sports Sciences, 22(1), 127–141.
54.Tarnopolsky, M. A. (2008). Effect of caffeine on the neuromuscular system—potential as an ergogenic aid. Applied Physiology, Nutrition, and Metabolism, 33(6), 1284–1289.
55.Tarnopolsky, M. A., Atkinson, S. A., MacDougall, J. D., Sale, D. G., & Sutton, J. R. (1989). Physiological responses to caffeine during endurance running in habitual caffeine users. Medicine and Science in Sports and Exercise, 21(4), 418–424.
56.Van Duinen, H., Lorist, M. M., & Zijdewind, I. (2005). The effect of caffeine on cognitive task performance and motor fatigue. Psychopharmacology, 180(3), 539-547.
57.Van Soeren, M. H., Sathasivam, P., Spriet, L. L., & Graham, T. E. (1993). Caffeine metabolism and epinephrine responses during exercise in users and nonusers. Journal of Applied Physiology, 75(2), 805–812.
58.Vanakoski, J., Kosunen, V., Meririnne, E., & Seppala, T. (1998). Creatine and caffeine in anaerobic and aerobic exercise: effects on physical performance and pharmacokinetic considerations. International Journal of Clinical Pharmacology and Therapeutics, 36(5), 258–262.
59.Vandenberghe, K., Gillis, N., Van Leemputte, M., Van Hecke, P., Vanstapel, F., & Hespel, P. (1996). Caffeine counteracts the ergogenic action of muscle creatine loading. Journal of Applied Physiology, 80(2), 452–457.
60.Wiles, J. D., Bird, S. R., Hopkins, J., & Riley, M. (1992). Effect of caffeinated coffee on running speed, respiratory factors, blood lactate and perceived exertion during 1500-m treadmill running. British Journal of Sports Medicine, 26(2), 116–120.