ISSN: 2450-8330
eISSN: 2353-3013
OAI
DOI: 10.18276/ab.2016.23-11
Issue archive /
No. 23
Values of B and T cells and their subpopulations in peripheral blood of rabbits
| Authors: |
Beata
Tokarz-Deptuła
Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland; Center of Molecular Biology and Biotechnology, Faculty of Biology, University of Szczecin, Szczecin, Poland Paulina Niedźwiedzka-Rystwej Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland, Center of Molecular Biology and Biotechnology, Faculty of Biology, University of Szczecin, Szczecin, Poland Alicja Trzeciak-Ryczek Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland; Center of Molecular Biology and Biotechnology, Faculty of Biology, University of Szczecin, Szczecin, Poland Andrzej Pępiak Domestic Animal Clinic, Łubinowa 17, Szczecin, Poland Wiesław Deptuła Department of Microbiology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin, Poland; Center of Molecular Biology and Biotechnology, Faculty of Biology, University of Szczecin, Szczecin, Poland |
| Keywords: | rabbit B and T lymphocytes reference values |
| Whole issue publication date: | 2016 |
| Page range: | 12 (127-138) |
Abstract
Determining the reference values for B and T lymphocytes and their subpopulations in rabbits used in Poland for economic and laboratory purposes is not very common. The goal of this study was to evaluate values of chosen B and T cells and their subpopulations in Polish mixed-breed rabbits, taking season of the year and sex of the animals into the consideration. The study was performed with the use of flow cytometry using monoclonal antibodies (mouse anti-rabbit). The study revealed that, both in Polish mixed-breed rabbits and in Polish mixed-breed rabbits with an addition of blood of meat breeds, the season of the year and sex of the animals affectthe percentage of B- and T-cells and their subpopulations in peripheral blood. As far as season of the year is concerned, it was shown that it mainly affects CD5+ T cells in mixed-breed rabbits and CD25+ T cells in mixed-breed rabbits with an addition of blood of meat breed, while taking sex of the animals into account, more changes were recorded in females in both groups of rabbits. Due to the fact that this is the first paper on those values in Polish rabbits conducted on a large amount of animals, the values may be treated as reference.
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Bibliography
| 1. | Anon. 1987. Information and training materials of the Laboratory Animals Section, General Assembly of the Association of Agricultural Engineers and Technicians, 187, pp. 26–77. |
| 2. | Anon. 2006. Regulation of the Minister of Agriculture and Rural Development of 10.03.2006 on detailed conditions for maintenance of laboratory animals in experimental units, breeding units and suppliers (Polish Journal of Laws of 2006, No. 50, item 368). |
| 3. | Archetti I., Tittarelli C., Ceriolo M., Brivio R., Grilli G., Lavazza A. 2008. Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial farms in Northern Italy. Proceed 9th World Rabbit Congress, Verona, Italy, pp. 1147–1151. |
| 4. | Bortolotti A., Castelli D., Bonnatti E. 1989. Hematology and serum chemistry values of adult, pregnant and newborn new Zealand rabbits (Oryctolagus cuniculus). Lab. Anim. Sci., 39: 437–439. |
| 5. | Chomicz L. 1967. Pathological changes of blood depending on sex and age of rabbits experimentally infected with sheep strain of Strongyloides papillosus. Acta Parasitol. Pol., 14: 251–263. |
| 6. | Chineke C.A., Ologun A.G., Ikeobi C.O.N. 2006. Haematological parameters in rabbits breeds and crosses in humid tropics. Pak. J. Biol. Sci., 9: 2102–2106. |
| 7. | Çetin N., Bekyürek T., Çetin E. 2009. Effects of sex, pregnancy and season on some haematological and biochemical blood values in angora rabbits. Scand. J. Lab. Anim. Sci., 36: 155–162. |
| 8. | Deptuła W., Górecka-Odkała D., Tokarz-Deptuła B. 1995. Dynamics of selected immunological parameters in 3-5 months old rabbits. Med. Weter., 51: 552–554. |
| 9. | Deptuła W., Kostrzewa A., Stosik M., Tokarz-Deptuła B., Wiktorowicz K. 1998. Subpopulations of peripheral blood lymphocytes in rabbits. Nowiny Lek., 67: 377–382. |
| 10. | Deptuła W., Niedźwiedzka-Rystwej P., Śliwa J., Tokarz-Deptuła B., Hukowska-Szematowicz B., Pawlikowska M. 2009. Specific immunity in healthy rabbits. Centr. Europ. J. Immunol., 34: 18–19. |
| 11. | Ezema W.S., Omeke B.C.O., Eze J.I., Nwanta J.A. 2009. Studies on the sexual behaviour, haematology and spermatogenesis of male rabbits infected with Trypanosoma brucei brucei. Comp. Clin. Pathol., 18: 63–68. |
| 12. | Ferrian S., Guerrero I., Blas E., García-Diego F., Viana D., Pascual J.J., Corpa J.M. 2012. How selection for reproduction or foundation for longevity could have affected blood lymphocyte populations of rabbit does under conventional and heat stress conditions. Vet. Immunol. Immunopath., 150: 53–60. |
| 13. | Jain J.M. 1994. Comparative hematologic features of some avian and mammalian species. In: Essentials of veterinary hematology. N.C. Jain (ed.). Philadelphia, Lea and Febiger, pp. 367–376. |
| 14. | Jeklova E., Leva L., Faldyna M. 2007. Lymphoid organ development in rabbits: major lymphocyte subsets Develop. Comp. Immunol., 31: 632–644. |
| 15. | Jeklova E., Leva L., Knotogova P., Faldyna M. 2009. Age-related changes in selected haematology parameters in rabbits. Res. Vet. Sci., 86: 525–528. |
| 16. | Laird C.W., Fox R.R., Mitchell B.P., Blau E.M., Schultz H.S. 1970. Effect of strain and age on some hematological parameters in the rabbit. Am. J. Physiol., 218: 1613–1617. |
| 17. | Martinec M., Härtlova H., Chodová D., Tůmová E., Fučiková A. 2012. Selected haematological and biochemical indicators in different breeds of rabbits. Acta Vet. Brno, 81: 371–375. |
| 18. | Nowaczyk P., Deptuła W., Tokarz-Deptuła B., Ossowski A., Suproń M. 2004. Dynamics of chosen immune-haematological parameters in rabbits in annual cycle. Centaur Lubuski, 61: 6–10. |
| 19. | Olayemi F.O., Nottidge H.O. 2007. Effect on the blood profiles of the New Zealand rabbit in Nigeria. Afr. J. Biomed. Res., 10: 73–76. |
| 20. | Pinna Pintor P., Grassini V. 1957. Individual and seasonal spontaneous variations of haematological values in normal male rabbits. Statistical survey. Acta Haematol. 17: 122–128. |
| 21. | Poljičak-Milas N., Kardum-Skelin I., Vudan M., Marenjak T.S., Ballarin-Perharić A., Milas Z. 2009. Blood cell count analyses and erythrocyte morphometry in New Zealand white rabbits. Veterinarski Arhiv, 79: 561–571. |
| 22. | Rohilla P.P., Bujaubauah K.M., Singh G., Kumar M. 2000. Haematological and biochemical studies on pure and crossbred rabbits. Ind. J. Anim. Sci., 70: 60–62. |
| 23. | Sabolovič N., Sabolovič D., Guilman A.M. 1977. T and B cell surface markers on rabbit lymphocytes. Immunol., 32: 581–590. |
| 24. | Tokarz-Deptuła B., Deptuła W. 2005. Values of selected immune and haematological parameters in healthy rabbits. Pol. J. Vet. Sci., 8: 107–112. |
| 25. | Tokarz-Deptuła B., Niedźwiedzka-Rystwej P., Adamiak M., Hukowska-Szematowicz B., Trzeciak-Ryczek A., Deptuła W. 2014. Values of white and red blood cell parameters in Polish mixed breed rabbits in the annual cycle. Pol. J. Vet. Sci., 17: 643–655. |
| 26. | Yaqub L.S., Kawu M.U., Ayo J.O. 2013. Influence of reproductive cycle, sex, age and season on haematologic parameters in domestic animals: a review. J. Cell Anim. Biol., 7: 37–43. |